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ISAC Test: 112 Allergy Tests in one tiny blood sample!

A Private Allergy ISAC test can test for 112 allergies from one tiny blood sample!

The ISAC test is a blood test that uses microchip technology to detect specific IgE antibodies to food and airborne allergens (this test uses method called ELISA with a special reader; it replaced the test previously called RAST). It allows  simultaneous measurement of specific antibodies to multiple allergen components in a single test, using only a few µl of serum or plasma. The allergens are pre-selected and include the most relevant specie specific and cross-reactive markers.

ISAC test London Allergy and Immunology Centre

A combination of innovative biochip technology with cutting edge research in molecular allergology has resulted in ImmunoCAP® ISAC – the most advanced in vitro diagnostic test for simultaneous measurement of specific IgE antibodies to a broad spectrum of allergen components.

This test is now available all over the UK in England, Scotland, Wales and Northern Ireland as well as for some international customers with the new self finger prick collection and sample in the post technology.

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Allergy testing is easy now there is even no need to travel to the clinic to have this test done!

You will receive a report with interpretation of your results from one of our Allergy and Immunology consultants!

Please e-mail us for details

In London you can attend our adult or paediatric allergy clinic or laboratory where a blood sample can be taken.

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The specific IgE chip delivers results for over a hundred components from more than 50 allergen sources

(see the list of allergens below).

The ImmunoCAP® ISAC test provides a large amount of allergen specific IgE  antibody information – the big picture – in a single step. This enables
patient investigations that require less time and resources. As a result, effective and optimized management can be started earlier, which in turn leads to improved patient health and quality of life. This test is relatively inexpensive comparing with the price of testing of sIgE to allergens individually.

How to have this test done:

If you are in London and want to be seen by an allergy consultant, or if you want your child or baby to be seen by our paediatric allergy consultant and want to ask the doctor to consider allergy testing blood test, please book an appointment on-line or call 020 314 33449.

If you live at a distance from London, or for any reason cannot attend face-to face appointment, please start with filling in ISAC allergy symptoms questionnaire, which will be reviewed by one of our allergy consultants. Please proceed to bank transfer payment of the invoice that you will receive by e-mail after you send your questionnaire. You will receive a sample collection pack from the lab (as shown in video) or if you opted for a collection in London you can attend the lab with printed request form for sample collection (the request form will be sent by e-mail after your payment).

Finger prick collection is not suitable for small children and babies, sample needs to be taken by a trained healthcare professional (our lab can provide necessary materials on request if you are located at a distance from London), or in London you can attend the lab with our form where the sample will be collected Mo-Fri 7am-7pm and Saturday 9am-5pm.

Your results will be ready in about 2 weeks (we aim 7 days). These will be reviewed by one of our allergy consultants in conjunction with your questionnaire, then sent to both you and to your GP (if you opted for this in the questionnaire).

If you have any further questions, need , a consultation, treatment, or if any problems are identified throughout this testing process, we would be happy to book you an appointment to meet with one of our specialists.

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 List of allergens tested in one test from a single blood sample from a finger prick

Allergen ComponentAllergen SourceLatin NameProtein GroupOn Immunocap?
FOOD ALLERGENS   
nGal d 1EggGallus domesticusOvomucoidY
nGal d 2EggGallus domesticusOvalbuminY
nGal d 3EggGallus domesticusConalbumin/OvotransferrinY
nGal d 5CSA (Livetin)Gallus domesticusSerum albuminN
nBos d 4Cow’s milkBos domesticusAlpha-lactalbuminY
nBos d 5Cow’s milkBos domesticusBeta-lactoglobulinY
nBos d 6BSABos domesticusSerum albuminY
nBos d 8Cow’s milkBos domesticusCaseinY
nBos d lactoferrinCow’s milkBos domesticusTransferrinY
rGad c 1CodGadus callariasParvalbuminY
nPen m 1ShrimpPenaeus monodonTropomyosinN
nPen m 2ShrimpPenaeus monodonArginine kinaseN
nPen m 4ShrimpPenaeus monodonSarcoplasmic Ca-binding proteinN
rAna o 2Cashew nutAnacardium occidentaleStorage protein, 11S globulinN
rBer e 1Brazil nutBertholletia excelsaStorage protein, 2S albuminY
rCor a 1.0401HazelnutCorylus avellanaPR-10 proteinY
rCor a 8HazelnutCorylus avellanaLipid transfer protein (nsLTP)Y
nCor a 9HazelnutCorylus avellanaStorage protein, 11S globulinN
nJug r 1WalnutJuglans regiaStorage protein, 2S albuminN
nJug r 2WalnutJuglans regiaStorage protein, 7S globulinN
nJug r 3WalnutJuglans regiaLipid transfer protein (nsLTP)N
nSes i 1Sesame seedSesamum indicumStorage protein, 2S albuminN
rAra h 1PeanutArachis hypogaeaStorage protein ,7S globulinY
rAra h 2PeanutArachis hypogaeaStorage protein, ConglutinY
rAra h 3PeanutArachis hypogaeaStorage protein, 11S globulinY
nAra h 6PeanutArachis hypogaeaStorage protein, ConglutinN
rAra h 8PeanutArachis hypogaeaPR-10 proteinY
rAra h 9PeanutArachis hypogaeaLipid transfer protein (nsLTP)Y
rGly m 4SoybeanGlycine maxPR-10 proteinY
nGly m 5SoybeanGlycine maxStorage protein, Beta-conglycininY
nGly m 6SoybeanGlycine maxStorage protein, GlycininY
nFag e 2BuckwheatFagopyrum esculentumStorage protein, 2S albuminN
rTri a 14WheatTriticum aestivumLipid transfer protein (nsLTP)N
rTri a 19.0101WheatTriticum aestivumCrude gliadinY
nTri a aA_TIWheatTriticum aestivumOmega-5 gliadinN
nAct d 1KiwiActinidia deliciosa N
nAct d 2KiwiActinidia deliciosaThaumatine-like proteinN
nAct d 5KiwiActinidia deliciosa N
rAct d 8KiwiActinidia deliciosaPR-10 proteinY
rApi g 1CeleryApium graveolensPR-10 proteinY
rMal d 1AppleMalus domesticaPR-10 proteinN
rPru p 1PeachPrunus persicaPR-10 proteinY
rPru p 3PeachPrunus persicaLipid transfer protein (nsLTP)Y
     
AEROALLERGENS   
nCyn d 1Bermuda grassCynodon dactylonGrass group 1Y
rPhl p 1Timothy grassPhleum pratenseGrass group 1Y
rPhl p 2Timothy grassPhleum pratenseGrass group 2Y
nPhl p 4Timothy grassPhleum pratense Y
rPhl p 5Timothy grassPhleum pratenseGrass group 5Y
rPhl p 6Timothy grassPhleum pratense Y
rPhl p 7Timothy grassPhleum pratensePolcalcinY
rPhl p 11Timothy grassPhleum pratense Y
rPhl p 12Timothy grassPhleum pratenseProfilinY
rAln g 1AlderAlnus glutinosaPR-10 proteinN
rBet v 1BirchBetula verrucosaPR-10 proteinY
rBet v 2BirchBetula verrucosaProfilinY
rBet v 4BirchBetula verrucosaPolcalcin, Calcium bonding proteinY
rCor a 1.0101Hazel pollenCorylus avellanaPR-10 proteinN
nCry j 1Japanese cederCryptomeria japonica N
nCup a 1CypressCupressus arizonica Y
nOle e 1OliveOlea europaea Y
nOle e 7OliveOlea europaeaLipid transfer protein (nsLTP)N
rOle e 9OliveOlea europaea N
rPla a 1Plane treePlatanus acerifolia N
nPla a 2Plane treePlatanus acerifolia N
rPla a 3Plane treePlatanus acerifoliaLipid transfer protein (nsLTP)N
nAmb a 1RagweedAmbrosia artemisiifolia Y
nArt v 1MugwortArtemisia vulgaris Y
nArt v 3MugwortArtemisia vulgarisLipid transfer protein (nsLTP)Y
rChe a 1GoosefootChenopodium album N
rMer a 1Annual mercuryMercurialis annuaProfilinN
rPar j 2Wall pellitoryParietaria judaicaLipid transfer protein (nsLTP)Y
rPla l 1Plantain (English)Plantago lanceolata N
nSal k 1SaltwortSalsola kali Y
rCan f 1DogCanis familiarisLipocalinY
rCan f 2DogCanis familiarisLipocalinY
nCan f 3DogCanis familiarisSerum albuminY
rCan f 5DogCanis familiarisArginine esteraseN
rEqu c 1HorseEquus caballusLipocalinN
nEqu c 3HorseEquus caballusSerum albuminN
rFel d 1CatFelis domesticusUteroglobinY
nFel d 2CatFelis domesticusSerum albuminY
rFel d 4CatFelis domesticusLipocalinN
nMus m 1MouseMus musculusLipocalinN
rAlt a 1AlternariaAlternaria alternata Y
rAlt a 6AlternariaAlternaria alternataEnolaseN
rAsp f 1AspergillusAspergillus fumigatus Y
rAsp f 3AspergillusAspergillus fumigatus Y
rAsp f 6AspergillusAspergillus fumigatusMn superoxide dismutaseY
rCla h 8CladosporiumCladosporium herbarum N
rBlo t 5House dust miteBlomia tropicalis N
nDer f 1House dust miteDermatophagoides farinae N
rDer f 2House dust miteDermatophagoides farinae Y
nDer p 1House dust miteDermatophagoides pteronyssinus Y
rDer p 2House dust miteDermatophagoides pteronyssinus Y
rDer p 10House dust miteDermatophagoides pteronyssinusTropomyosinY
rLep d 2Storage miteLepidoglyphus destructor N
rBla g 1CockroachBlattella germanica N
rBla g 2CockroachBlattella germanica N
rBla g 5CockroachBlattella germanica N
nBla g 7CockroachBlattella germanicaTropomyosinN
     
OTHER   
rApi m 1Honey bee venomApis melliferaPhospholipase A2Y
nApi m 4Honey bee venomApis melliferaMelittinN
rPol d 5Paper wasp venomPolistes dominulusVenom, Antigen 5Y
rVes v 5Common wasp venomVespula vulgarisVenom, Antigen 5Y
rAni s 1AnisakisAnisakis simplex N
rAni s 3AnisakisAnisakis simplexTropomyosinN
rHev b 1LatexHevea brasiliensis Y
rHev b 3LatexHevea brasiliensis Y
rHev b 5LatexHevea brasiliensis Y
rHev b 6.01LatexHevea brasiliensis Y
rHev b 8Latex ProfilinY
nMUXF3Sugar epitope from Bromelain CCD-markerY

Storage protein

• Proteins stable to heat and digestion causing reactions also to  cooked foods.
• often associated with systemic and more severe reactions in addition to OAS.
• Proteins found in nuts and seeds serving as source material during  the growth of a new plant.

LTP (non-speciἀc Lipid Transfer Protein, nsLTP)

• Proteins stable to heat and digestion causing reactions also to  cooked foods.
• often associated with systemic and more severe reactions in addition  to OAS.
• Associated with allergic reactions to fruit and vegetables especially  in regions where peach and closely related fruits are cultivated.

Pr-10 protein, Bet v 1 homologue

• most PR-10 proteins are sensitive to heat and digestion and cooked foods are often tolerated.
• often associated with local symptoms such as oral allergy syndrome (OAS).
• Associated with allergic reactions to pollens, fruits and vegetables.

Proflin

• Proteins sensitive to heat and digestion and cooked foods are often tolerated.
•  Seldom associated with clinical symptoms but may cause local and even severe reactions in some patients.
• Profilins ae present in all pollen and plant foods.

CCD

• A marker for sensitization to cross-reactive carbohydrate determinants.
• Rarely causes allergic reactions, but may produce positive in-vitro test results to CCd-containing allergens from pollen, plant foods, insects  and venoms.

Tropomyosin

• Proteins stable to heat and digestion causing reactions also to cooked foods. • As food allergen often associated with systemic and more severe reactions in addition to OAS.
• Actin-binding proteins in muscle fibres and a marker for cross-reactivity between crustaceans, mites and cockroach.

Parvalbumin

• Proteins stable to heat and digestion causing reactions also to cooked foods.
• often associated with systemic and more severe reactions in addition to OAS.
• major allergens in fish and a marker for cross-reactivity among different species of fish and amphibian.

Serum albumin

• Proteins fairly sensitive to heat and digestion.
• Proteins present in different biological fluids and solids in all animals e.g.: cow’s milk, blood, beef and epithelia.
• Cross-reactions between albumins from different mammalian species are well known, for example between cat and dog and cat and pig (pork).

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